Ontology highlight
ABSTRACT:
SUBMITTER: Sawin KE
PROVIDER: S-EPMC2810510 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
Sawin Kenneth E KE Bicho Claudia C CC Snaith Hilary A HA
Analytical biochemistry 20090930 2
Immunoglobulin G (IgG)-Sepharose is often used for purification of protein A- and tandem affinity purification (TAP)-tagged proteins from eukaryotic cells, but because it is based on an agarose matrix, it is not always optimal for all proteins. Synthetic matrices such as IgG-Dynabeads have improved properties over IgG-Sepharose but are generally expensive. Here we describe the preparation and properties of an IgG matrix based on Fractogel EMD beads. As a synthetic-based matrix, IgG-Fractogel has ...[more]