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Panel of prototypical raltegravir-resistant infectious molecular clones in a novel integrase-deleted cloning vector.

ABSTRACT: We created an HIV-1 cloning vector, pNL4.3DeltaIN, to generate recombinant infectious molecular clones for analysis of patient-derived HIV-1 integrase coding regions. Using this vector, we constructed a panel of clinically derived viruses with the canonical patterns of raltegravir resistance mutations and submitted the panel to the NIH AIDS Research and Reference Reagent Program. Investigational integrase inhibitors with activity against these clones are likely to retain activity against the most clinically relevant raltegravir-resistant variants.

SUBMITTER: Reuman EC

PROVIDER: S-EPMC2812164 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature

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Panel of prototypical raltegravir-resistant infectious molecular clones in a novel integrase-deleted cloning vector.

Reuman Elizabeth C EC Bachmann Michael H MH Varghese Vici V Fessel W Jeffrey WJ Shafer Robert W RW

Antimicrobial agents and chemotherapy 20091116 2

We created an HIV-1 cloning vector, pNL4.3DeltaIN, to generate recombinant infectious molecular clones for analysis of patient-derived HIV-1 integrase coding regions. Using this vector, we constructed a panel of clinically derived viruses with the canonical patterns of raltegravir resistance mutations and submitted the panel to the NIH AIDS Research and Reference Reagent Program. Investigational integrase inhibitors with activity against these clones are likely to retain activity against the mos ...[more]

PMID: 19917747

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