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Genetic analysis for the lack of expression of the O157 antigen in an O Rough:H7 Escherichia coli strain.


ABSTRACT: The O-antigen (rfb) operon and related genes of MA6, an O rough:H7 Shiga-toxigenic Escherichia coli strain, were examined to determine the cause of the lack of O157 expression. A 1,310-bp insertion, homologous to IS629, was observed within its gne gene. trans complementation with a functional gne gene from O157:H7 restored O157 antigen expression in MA6.

SUBMITTER: Rump LV 

PROVIDER: S-EPMC2813021 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature

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Genetic analysis for the lack of expression of the O157 antigen in an O Rough:H7 Escherichia coli strain.

Rump Lydia V LV   Feng Peter C H PC   Fischer Markus M   Monday Steven R SR  

Applied and environmental microbiology 20091130 3


The O-antigen (rfb) operon and related genes of MA6, an O rough:H7 Shiga-toxigenic Escherichia coli strain, were examined to determine the cause of the lack of O157 expression. A 1,310-bp insertion, homologous to IS629, was observed within its gne gene. trans complementation with a functional gne gene from O157:H7 restored O157 antigen expression in MA6. ...[more]

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2012-01-06 | GSE30893 | GEO