Ontology highlight
ABSTRACT:
SUBMITTER: de Hoon MJ
PROVIDER: S-EPMC2813481 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
de Hoon Michiel J L MJ Taft Ryan J RJ Hashimoto Takehiro T Kanamori-Katayama Mutsumi M Kawaji Hideya H Kawano Mitsuoki M Kishima Mami M Lassmann Timo T Faulkner Geoffrey J GJ Mattick John S JS Daub Carsten O CO Carninci Piero P Kawai Jun J Suzuki Harukazu H Hayashizaki Yoshihide Y
Genome research 20100105 2
MicroRNAs (miRNAs) are short (20-23 nt) RNAs that are sequence-specific mediators of transcriptional and post-transcriptional regulation of gene expression. Modern high-throughput technologies enable deep sequencing of such RNA species on an unprecedented scale. We find that the analysis of small RNA deep-sequencing libraries can be affected by cross-mapping, in which RNA sequences originating from one locus are inadvertently mapped to another. Similar to cross-hybridization on microarrays, cros ...[more]