Ontology highlight
ABSTRACT:
SUBMITTER: Williams GJ
PROVIDER: S-EPMC2813856 | biostudies-literature | 2008 Apr
REPOSITORIES: biostudies-literature
Williams Gavin J GJ Goff Randal D RD Zhang Changsheng C Thorson Jon S JS
Chemistry & biology 20080401 4
A comprehensive two-phase "hot spot" saturation mutagenesis strategy for the rapid evolution of glycosyltransferase (GT) specificity for nonnatural acceptors is described. Specifically, the application of a high-throughput screen (based on the fluorescent acceptor umbelliferone) was used to identify key amino acid hot spots that contribute to GT proficiency and/or promiscuity. Saturation mutagenesis of the corresponding hot spots facilitated the utilization of a lower-throughput screen to provid ...[more]