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Osteogenic differentiation of human amniotic fluid-derived stem cells induced by bone morphogenetic protein-7 and enhanced by nanofibrous scaffolds.

ABSTRACT: Amniotic fluid-derived stem cells (AFSCs) are becoming an important source of cells for regenerative medicine given their apparent advantages of accessibility, renewal capacity and multipotentiality. In the intermediate stage between the embryonic stem cells (ESCs) and adult stem cells, AFSCs may have a distinct mechanism to choose their fate. Unfortunately, until now, little is known about how bone morphogenetic proteins (BMPs) control the osteoblastic differentiation of AFSCs, especially on 3D scaffolds. Our research shows that human AFSCs (hAFSCs) can be induced for osteoblastic differentiation by rhBMP-7, and hAFSCs respond to rhBMP-7 more strongly than human mesenchymal stem cells (hMSCs). As synthetic ECM, scaffolds play a central role in tissue engineering. The hAFSCs, on the nanofibrous scaffolds (NF scaffolds) with morphology similar to that of natural collagen fibers, showed significantly enhanced alkaline phosphatase (ALP) activity, calcium content, von Kossa staining and the expression of osteogenic genes than those on the traditional scaffolds, i.e. solid walled scaffolds. The data on the bone formation in vivo presented further evidence that biomimetic NF scaffolds provided hAFSCs a more favorable synthetic ECM, and thus, facilitated the osteogenic differentiation of hAFSCs. The relative strong responsiveness to rhBMP-7 makes hAFSCs promising in bone regeneration. The synthetic NF scaffolds, which mimic the morphology of natural collagen fibers, enhanced the osteoblastic differentiation of hAFSCs in vitro and bone formation in vivo.

SUBMITTER: Sun H

PROVIDER: S-EPMC2813991 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature

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Osteogenic differentiation of human amniotic fluid-derived stem cells induced by bone morphogenetic protein-7 and enhanced by nanofibrous scaffolds.

Sun Hongli H Feng Kai K Hu Jiang J Soker Shay S Atala Anthony A Ma Peter X PX

Biomaterials 20091025 6

Amniotic fluid-derived stem cells (AFSCs) are becoming an important source of cells for regenerative medicine given their apparent advantages of accessibility, renewal capacity and multipotentiality. In the intermediate stage between the embryonic stem cells (ESCs) and adult stem cells, AFSCs may have a distinct mechanism to choose their fate. Unfortunately, until now, little is known about how bone morphogenetic proteins (BMPs) control the osteoblastic differentiation of AFSCs, especially on 3D ...[more]

PMID: 19857889

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Project description:Bone disorders are major health issues requiring specialized care; however, the traditional bone grafting method had several limitations. Thus, bone tissue engineering has become a potential alternative. In therapeutic treatments, using fetal bovine serum (FBS) as a culture supplement may result in the risk of contamination and host immunological response; therefore, human platelet lysate (hPL) has been considered a viable alternative source. This study attempted to compare the effectiveness and safety of different culture supplements, either FBS or hPL, on the osteoblastic differentiation potential of mesenchymal stem cells derived from human amniotic fluid (hAF-MSCs) under a three-dimensional gelatin scaffold. The results indicate that hAF-MSCs have the potential to be used in clinical applications as they meet the criteria for mesenchymal stem cells based on their morphology, the expression of a particular surface antigen, their proliferation ability, and their capacity for multipotent differentiation. After evaluation by MTT and Alamar blue proliferation assay, 10% of hPL was selected. The osteogenic differentiation of hAF-MSCs under three-dimensional gelatin scaffold using osteogenic-induced media supplemented with hPL was achievable and markedly stimulated osteoblast differentiation. Moreover, the expressions of osteoblastogenic related genes, including OCN, ALP, and COL1A1, exhibited the highest degree of expression under hPL-supplemented circumstances when compared with the control and the FBS-supplemented group. The induced cells under hPL-supplemented conditions also presented the highest ALP activity level and the greatest degree of calcium accumulation. These outcomes would indicate that hPL is a suitable substitute for animal derived serum. Importantly, osteogenic differentiation of human amniotic fluid derived mesenchymal stem cells using hPL-supplemented media and three-dimensional scaffolds may open the door to developing an alternative construct for repairing bone defects.

Dataset Information

Osteogenic differentiation of human amniotic fluid-derived stem cells induced by bone morphogenetic protein-7 and enhanced by nanofibrous scaffolds.

Publications

Osteogenic differentiation of human amniotic fluid-derived stem cells induced by bone morphogenetic protein-7 and enhanced by nanofibrous scaffolds.

Similar Datasets

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