Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Functionalized carbon nanotubes specifically bind to alpha-chymotrypsin's catalytic site and regulate its enzymatic function.

ABSTRACT: Although carbon nanotubes (CNTs) have been shown to nonspecifically bind proteins through charge complementary, pi-pi stacking or hydrophobic interactions, they have not been shown to bind to a specific site on proteins. By generating surface molecular diversity, we created functionalized CNTs that recognize and bind to the catalytic site of alpha-chymotrypsin and inhibit its enzymatic activity competitively.

SUBMITTER: Zhang B

PROVIDER: S-EPMC2814308 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Functionalized carbon nanotubes specifically bind to alpha-chymotrypsin's catalytic site and regulate its enzymatic function.

Zhang Bin B Xing Yuehan Y Li Zhenwei Z Zhou Hongyu H Mu Qingxin Q Yan Bing B

Nano letters 20090601 6

Although carbon nanotubes (CNTs) have been shown to nonspecifically bind proteins through charge complementary, pi-pi stacking or hydrophobic interactions, they have not been shown to bind to a specific site on proteins. By generating surface molecular diversity, we created functionalized CNTs that recognize and bind to the catalytic site of alpha-chymotrypsin and inhibit its enzymatic activity competitively. ...[more]

PMID: 19408924

Similar Datasets

Enzymatic degradation of multiwalled carbon nanotubes.

Project description:Because of their unique properties, carbon nanotubes and, in particular, multiwalled carbon nanotubes (MWNTs) have been used for the development of advanced composite and catalyst materials. Despite their growing commercial applications and increased production, the potential environmental and toxicological impacts of MWNTs are not fully understood; however, many reports suggest that they may be toxic. Therefore, a need exists to develop protocols for effective and safe degradation of MWNTs. In this article, we investigated the effect of chemical functionalization of MWNTs on their enzymatic degradation with horseradish peroxidase (HRP) and hydrogen peroxide (H(2)O(2)). We investigated HRP/H(2)O(2) degradation of purified, oxidized, and nitrogen-doped MWNTs and proposed a layer-by-layer degradation mechanism of nanotubes facilitated by side wall defects. These results provide a better understanding of the interaction between HRP and carbon nanotubes and suggest an eco-friendly way of mitigating the environmental impact of nanotubes.

| S-EPMC3113459 | biostudies-literature

Globular Shaped Polypyrrole Doped Well-Dispersed Functionalized Multiwall Carbon Nanotubes/Nafion Composite for Enzymatic Glucose Biosensor Application.

Project description:Herein, we report preparation of a bio-nanohybrid material of homogenously dispersed functionalized multiwall carbon nanotubes (fMWCNTs) in Nafion (Nf) doped with polypyrrole (PPy) and followed by one-step in situ electrochemical polymerization along with glucose oxidase (GOx) on a platinum (Pt) electrode. The bioengineered Nf-GOx-fMWCNTs-PPy/Pt electrode showed excellent electrocatalytic performance to detect glucose with a high sensitivity (54.2 μAmM-1 cm-2) in linear range of up to 4.1 mM as well as a low detection limit of 5 μM (S/N = 3), response time within 4 s, good selectivity, stability, and practical applicability. It is our hope that the comprehensive results will contribute to design an efficient glucose biosensor with practical prospects for biomedical applications.

| S-EPMC5701076 | biostudies-literature

Deploying RNA and DNA with Functionalized Carbon Nanotubes.

Project description:Carbon nanotubes internalize into cells and are potential molecular platforms for siRNA and DNA delivery. A comprehensive understanding of the identity and stability of ammoniumfunctionalized carbon nanotube (f-CNT)-based nucleic acid constructs is critical to deploying them in vivo as gene delivery vehicles. This work explored the capability of f-CNT to bind single- and double-strand oligonucleotides by determining the thermodynamics and kinetics of assembly and the stoichiometric composition in aqueous solution. Surprisingly, the binding affinity of f-CNT and short oligonucleotide sequences was in the nanomolar range, kinetics of complexation were extremely rapid, and from one to five sequences were loaded per nanotube platform. Mechanistic evidence for an assembly process that involved electrostatic, hydrogen-bonding and ?-stacking bonding interactions was obtained by varying nanotube functionalities, oligonucleotides, and reaction conditions. 31P-NMR and spectrophotometric fluorescence emission data described the conditions required to assemble and stably bind a DNA or RNA cargo for delivery in vivo and the amount of oligonucleotide that could be transported. The soluble oligonucleic acid-f-CNT supramolecular assemblies were suitable for use in vivo. Importantly, key evidence in support of an elegant mechanism by which the bound nucleic acid material can be 'off-loaded' from the f-CNT was discovered.

| S-EPMC3634719 | biostudies-literature

Self-assembled functionalized graphene nanoribbons from carbon nanotubes.

Project description:Graphene nanoribbons (GNR) were generated in ethanol solution by unzipping pyrrolidine-functionalized carbon nanotubes under mild conditions. Evaporation of the solvent resulted in regular few-layer stacks of graphene nanoribbons observed by transmission electron microscopy (TEM) and X-ray diffraction. The experimental interlayer distance (0.49-0.56?nm) was confirmed by computer modelling (0.51?nm). Computer modelling showed that the large interlayer spacing (compared with graphite) is due to the presence of the functional groups and depends on their concentration. Stacked nanoribbons were observed to redissolve upon solvent addition. This preparation method could allow the fine-tuning of the interlayer distances by controlling the number and/or the nature of the chemical groups in between the graphene layers.

| S-EPMC4420582 | biostudies-literature

Functionalized multiwalled carbon nanotubes as ultrasound contrast agents.

Project description:Ultrasonography is a fundamental diagnostic imaging tool in everyday clinical practice. Here, we are unique in describing the use of functionalized multiwalled carbon nanotubes (MWCNTs) as hyperechogenic material, suggesting their potential application as ultrasound contrast agents. Initially, we carried out a thorough investigation to assess the echogenic property of the nanotubes in vitro. We demonstrated their long-lasting ultrasound contrast properties. We also showed that ultrasound signal of functionalized MWCNTs is higher than graphene oxide, pristine MWCNTs, and functionalized single-walled CNTs. Qualitatively, the ultrasound signal of CNTs was equal to that of sulfur hexafluoride (SonoVue), a commercially available contrast agent. Then, we found that MWCNTs were highly echogenic in liver and heart through ex vivo experiments using pig as an animal model. In contrast to the majority of ultrasound contrast agents, we observed in a phantom bladder that the tubes can be visualized within a wide variety of frequencies (i.e., 5.5-10 MHz) and 12.5 MHz using tissue harmonic imaging modality. Finally, we demonstrated in vivo in the pig bladder that MWCNTs can be observed at low frequencies, which are appropriate for abdominal organs. Importantly, we did not report any toxicity of CNTs after 7 d from the injection by animal autopsy, organ histology and immunostaining, blood count, and chemical profile. Our results reveal the enormous potential of CNTs as ultrasound contrast agents, giving support for their future applications as theranostic nanoparticles, combining diagnostic and therapeutic modalities.

| S-EPMC3478634 | biostudies-literature

Electro-oxidation of amino-functionalized multiwalled carbon nanotubes.

Project description:We report the electrochemistry of amino-functionalized multiwalled carbon nanotubes (MWCNTs-NH2) in the pH range from 0.3 to 6.4 using quantitative cyclic voltammetry (CV) and single entity electrochemistry measurements, making comparison with non-functionalized MWCNTs. CV showed the latter to both catalyze the solvent (water) decomposition and to undergo irreversible electro-oxidation forming oxygen containing surface functionality. The MWCNTs-NH2 additionally undergo an irreversible oxidation to an extent which is dependent on the pH of the solution, reflecting the variable amount of deprotonated amino groups present as a function of pH. Nano-impact experiments conducted at the single particle level confirmed the oxidation of both types of MWCNTs, showing agreement with the CV. The pK a of the amino groups in MWCNTs was determined via both electrochemical methods giving consistent values of ca. 2.5.

| S-EPMC8809411 | biostudies-literature

Transcriptomic study of NR8383 rat macrophages cells following exposure to COOH-functionalized carbon nanotubes (NRCWE042)

Project description:To identify differentially expressed genes and key biological pathways that define toxicity following nanomaterial exposure, we performed microarray analyses on NR8383 macrophages exposed for 4 h to 6.8 cm²/cm² of COOH-functionalized carbon nanotubes (NRCWE042). This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement N°. 686098

2023-08-18 | GSE156660 | GEO

Transcriptomic study of NR8383 rat macrophages cells following exposure to NH2-functionalized carbon nanotubes (NRCWE049)

Project description:To identify differentially expressed genes and key biological pathways that define toxicity following nanomaterial exposure, we performed microarray analyses on NR8383 macrophages exposed for 4 h to 1.2 cm²/cm² of NH2-functionalized carbon nanotubes (NRCWE049). This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement N°. 686098

2023-08-18 | GSE156666 | GEO

Biodefunctionalization of functionalized single-walled carbon nanotubes in mice.

Project description:Chemically modified carbon nanotubes with hydrophilic functionalities such as polyethylene glycols (PEGs) are widely pursued for potential biological and biomedical applications. In this study, PEGylated single-walled carbon nanotubes (PEG-SWNT) were intravenously administrated into mice to study their biodefunctionalization in vivo by using complementary Raman and photoluminescence measurements. There was meaningful defunctionalization of PEG-SWNT in liver over time, but not in spleen under similar conditions. The evidence from spectroscopic characterization and analyses is presented, and mechanistic implications are discussed.

| S-EPMC2886730 | biostudies-literature

Giant spin signals in chemically functionalized multiwall carbon nanotubes.

Project description:Transporting quantum information such as the spin information over micrometric or even millimetric distances is a strong requirement for the next-generation electronic circuits such as low-voltage spin-logic devices. This crucial step of transportation remains delicate in nontopologically protected systems because of the volatile nature of spin states. Here, a beneficial combination of different phenomena is used to approach this sought-after milestone for the beyond-Complementary Metal Oxide Semiconductor (CMOS) technology roadmap. First, a strongly spin-polarized charge current is injected using highly spin-polarized hybridized states emerging at the complex ferromagnetic metal/molecule interfaces. Second, the spin information is brought toward the conducting inner shells of a multiwall carbon nanotube used as a confined nanoguide benefiting from both weak spin-orbit and hyperfine interactions. The spin information is finally electrically converted because of a strong magnetoresistive effect. The experimental results are also supported by calculations qualitatively revealing exceptional spin transport properties of this system.

| S-EPMC7399653 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data