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Intravital microscopy identifies selectins that regulate T cell traffic into allografts.

ABSTRACT: T cell homing to sites of injury and inflammation is a critical step for adaptive immune responses. While much has been learned regarding T cell homing to lymphoid tissues, few studies have directly observed trafficking events during an effector response. In this study, we developed a model that uses intravital fluorescence videomicroscopy to determine the molecules critical to T cell rolling within skin allograft microvasculature during the effector phase of the rejection response. Additional studies were performed to quantify T cell infiltrates as rejection progressed. We found that P-selectin and E-selectin expressed on postcapillary venules play overlapping roles in the recruitment of activated T cells in a SCID reconstitution model of skin graft rejection and are important in T cell accumulation at the graft site. Surprisingly, we also found that naive T cells are recruited and accumulate via constitutive T cell L-selectin and upregulated L-selectin ligands on rejecting allograft vasculature. These data indicated that a specific retinue of molecules is upregulated during the rejection response, and they suggest potential future therapeutic targets.

SUBMITTER: Jones TR

PROVIDER: S-EPMC281648 | biostudies-literature | 2003 Dec

REPOSITORIES: biostudies-literature

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Intravital microscopy identifies selectins that regulate T cell traffic into allografts.

Jones Thomas R TR Shirasugi Nozomu N Adams Andrew B AB Pearson Thomas C TC Larsen Christian P CP

The Journal of clinical investigation 20031201 11

T cell homing to sites of injury and inflammation is a critical step for adaptive immune responses. While much has been learned regarding T cell homing to lymphoid tissues, few studies have directly observed trafficking events during an effector response. In this study, we developed a model that uses intravital fluorescence videomicroscopy to determine the molecules critical to T cell rolling within skin allograft microvasculature during the effector phase of the rejection response. Additional s ...[more]

PMID: 14660747

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Intravital microscopy identifies selectins that regulate T cell traffic into allografts.

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Intravital microscopy identifies selectins that regulate T cell traffic into allografts.

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