Unknown

Dataset Information

0

Proton-detected heteronuclear single quantum correlation NMR spectroscopy in rigid solids with ultra-fast MAS.


ABSTRACT: In this article, we show the potential for utilizing proton-detected heteronuclear single quantum correlation (HSQC) NMR in rigid solids under ultra-fast magic angle spinning (MAS) conditions. The indirect detection of carbon-13 from coupled neighboring hydrogen nuclei provides a sensitivity enhancement of 3- to 4-fold in crystalline amino acids over direct-detected versions. Furthermore, the sensitivity enhancement is shown to be significantly larger for disordered solids that display inhomogeneously broadened carbon-13 spectra. Latrodectus hesperus (Black Widow) dragline silk is given as an example where the sample is mass-limited and the sensitivity enhancement for the proton-detected experiment is 8- to 13-fold. The ultra-fast MAS proton-detected HSQC solid-state NMR technique has the added advantage that no proton homonuclear decoupling is applied during the experiment. Further, well-resolved, indirectly observed carbon-13 spectra can be obtained in some cases without heteronuclear proton decoupling.

SUBMITTER: Holland GP 

PROVIDER: S-EPMC2818250 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Proton-detected heteronuclear single quantum correlation NMR spectroscopy in rigid solids with ultra-fast MAS.

Holland Gregory P GP   Cherry Brian R BR   Jenkins Janelle E JE   Yarger Jeffery L JL  

Journal of magnetic resonance (San Diego, Calif. : 1997) 20091004 1


In this article, we show the potential for utilizing proton-detected heteronuclear single quantum correlation (HSQC) NMR in rigid solids under ultra-fast magic angle spinning (MAS) conditions. The indirect detection of carbon-13 from coupled neighboring hydrogen nuclei provides a sensitivity enhancement of 3- to 4-fold in crystalline amino acids over direct-detected versions. Furthermore, the sensitivity enhancement is shown to be significantly larger for disordered solids that display inhomogen  ...[more]

Similar Datasets

| S-EPMC9040908 | biostudies-literature
| S-EPMC5771212 | biostudies-literature
| S-EPMC6668038 | biostudies-literature
| S-EPMC3915937 | biostudies-literature
| S-EPMC6252081 | biostudies-literature
| S-EPMC6088370 | biostudies-literature
| S-EPMC5764182 | biostudies-literature
| S-EPMC4156866 | biostudies-other
| S-EPMC8901535 | biostudies-literature
| S-EPMC5547042 | biostudies-literature