Ontology highlight
ABSTRACT:
SUBMITTER: Xu C
PROVIDER: S-EPMC2818787 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
Xu Chunying C Yu Zhuoxin Z Inouye Masayori M Brodsky Barbara B Mirochnitchenko Oleg O
Biomacromolecules 20100201 2
The presence of the (Gly-Xaa-Yaa)(n) open reading frames in different bacteria predicts the existence of an expanded family of collagen-like proteins. To further explore the triple-helix motif and stabilization mechanisms in the absence of hydroxyproline (Hyp), predicted novel collagen-like proteins from Gram-positive and -negative bacteria were expressed in Escherichia coli and characterized. Soluble proteins capable of successful folding and in vitro refolding were observed for collagen protei ...[more]