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Evaluation of biodistribution and safety of adenovirus vector containing MDR1 in mice.

ABSTRACT: BACKGROUND: The aim of this study is to examine the safety and distribution of Ad-EGFP-MDR1, an adenovirus encoding human multidurg resistance gene (human MDR1), in the mice colon carcinoma model. METHODS: After bone marrow cells (BMCs) were infected with Ad-EGFP-MDR1, they were administered by intra bone marrow-bone marrow transplantation (IBM-BMT). Total adenovirus antibody and serum adenovirus neutralizing factor (SNF) were determined. Biodistribution of Ad-EGFP-MDR1 was detected by in situ hybridization and immunohistochemistry. The peripheral hematocyte white blood cell (WBC), haemoglobin (Hb), red blood cell (RBC) and platelet (Plt) counts were analyzed. RESULTS: Neither total adenovirus antibody nor SNF increased weeks after BMT. In situ hybridization and immunohistochemistry demonstrated concordant expression of human MDR1 and P-gp which were found in lung, intestine, kidney and BMCs after BMT, but not detected in liver, spleen, brain and tumor. No significant abnormality of the recovery hematocyte was observed on Day 30 after treatment. CONCLUSION: The results indicate that IBM-BMT administration of a replication defective adenovirus is a feasible mode of delivery, allowing exogenous transference. The findings in this study are conducted for the future long-term studies of safety assessment of Ad-EGFP-MDR1.

SUBMITTER: Zhao Z

PROVIDER: S-EPMC2819043 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature

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Evaluation of biodistribution and safety of adenovirus vector containing MDR1 in mice.

Zhao ZhenZhen Z Liu Wei W Su Yuxi Y Zhu Jin J Zheng GaiHuan G Luo Qing Q Jin XianQing X

Journal of experimental & clinical cancer research : CR 20100104

<h4>Background</h4>The aim of this study is to examine the safety and distribution of Ad-EGFP-MDR1, an adenovirus encoding human multidurg resistance gene (human MDR1), in the mice colon carcinoma model.<h4>Methods</h4>After bone marrow cells (BMCs) were infected with Ad-EGFP-MDR1, they were administered by intra bone marrow-bone marrow transplantation (IBM-BMT). Total adenovirus antibody and serum adenovirus neutralizing factor (SNF) were determined. Biodistribution of Ad-EGFP-MDR1 was detected ...[more]

PMID: 20044941

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Evaluation of biodistribution and safety of adenovirus vector containing MDR1 in mice.

Publications

Evaluation of biodistribution and safety of adenovirus vector containing MDR1 in mice.

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