Protein interaction platforms: visualization of interacting proteins in yeast.
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ABSTRACT:
SUBMITTER: Schmitz AM
PROVIDER: S-EPMC2819136 | biostudies-literature | 2009 Jul
REPOSITORIES: biostudies-literature
Publications
Protein interaction platforms: visualization of interacting proteins in yeast.
Schmitz Alexa M AM Morrison Monica F MF Agunwamba Akochi O AO Nibert Max L ML Lesser Cammie F CF
Nature methods 20090531 7
Here we describe the protein interaction platform assay, a method for identifying interacting proteins in Saccharomyces cerevisiae. This assay relies on the reovirus scaffolding protein microNS, which forms large focal inclusions in living cells. When a query protein is fused to microNS and potential interaction partners are fused to a fluorescent reporter, interactors can be identified by screening for yeast that display fluorescent foci. ...[more]
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