Dataset Information

Proteomic analysis of interactions between a deep-sea thermophilic bacteriophage and its host at high temperature.

ABSTRACT: The virus-host interaction is essential to understanding the role that viruses play in ecological and geochemical processes in deep-sea vent ecosystems. Virus-induced changes in cellular gene expression and host physiology have been studied extensively. However, the molecular mechanism of interaction between a bacteriophage and its host at high temperature remains poorly understood. In the present study, the virus-induced gene expression profile of Geobacillus sp. E263, a thermophile isolated from a deep-sea hydrothermal ecosystem, was characterized. Based on proteomic analysis and random arbitrarily primed PCR (RAP-PCR) of Geobacillus sp. E263 cultured under non-bacteriophage GVE2 infection and GVE2 infection conditions, there were two types of protein/gene profiles in response to GVE2 infection. Twenty differentially expressed genes and proteins were revealed that could be grouped into 3 different categories based on cellular function, suggesting a coordinated response to infection. These differentially expressed genes and proteins were further confirmed by Northern blot analysis. To characterize the host proteins in response to virus infection, aspartate aminotransferase (AST) was inactivated to construct the AST mutant of Geobacillus sp. E263. The results showed that the AST protein was essential in virus infection. Thus, transcriptional and proteomic analyses and functional analysis revealed previously unknown host responses to deep-sea thermophilic virus infection.

SUBMITTER: Wei D

PROVIDER: S-EPMC2820902 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature

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Proteomic analysis of interactions between a deep-sea thermophilic bacteriophage and its host at high temperature.

Wei Dahai D Zhang Xiaobo X

Journal of virology 20091216 5

The virus-host interaction is essential to understanding the role that viruses play in ecological and geochemical processes in deep-sea vent ecosystems. Virus-induced changes in cellular gene expression and host physiology have been studied extensively. However, the molecular mechanism of interaction between a bacteriophage and its host at high temperature remains poorly understood. In the present study, the virus-induced gene expression profile of Geobacillus sp. E263, a thermophile isolated fr ...[more]

PMID: 20015994

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Dataset Information

Proteomic analysis of interactions between a deep-sea thermophilic bacteriophage and its host at high temperature.

Publications

Proteomic analysis of interactions between a deep-sea thermophilic bacteriophage and its host at high temperature.

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