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Optimization of RNA extraction for PCR quantification of aromatic compound degradation genes.

ABSTRACT: Seven different bacterial strains and primer sets and a mixed community were used to evaluate the use of reverse transcriptase quantitative PCR (Q-PCR) and Q-PCR of oxygenase genes to assess various approaches for monitoring the bioremediation of polluted sites. Differences in maximum activity were seen when different RNA extraction kits were compared.

SUBMITTER: Kong W

PROVIDER: S-EPMC2820950 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature

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Optimization of RNA extraction for PCR quantification of aromatic compound degradation genes.

Kong Weidong W Nakatsu Cindy H CH

Applied and environmental microbiology 20091218 4

Seven different bacterial strains and primer sets and a mixed community were used to evaluate the use of reverse transcriptase quantitative PCR (Q-PCR) and Q-PCR of oxygenase genes to assess various approaches for monitoring the bioremediation of polluted sites. Differences in maximum activity were seen when different RNA extraction kits were compared. ...[more]

PMID: 20023086

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