Ontology highlight
ABSTRACT:
SUBMITTER: Lakshman N
PROVIDER: S-EPMC2822042 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
Experimental eye research 20091216 2
The purpose of this study was to assess quantitatively the differences in morphology, cytoskeletal organization and mechanical behavior between quiescent corneal keratocytes and activated fibroblasts in a 3-D culture model. Primary cultures of rabbit corneal keratocytes and fibroblasts were plated inside type I collagen matrices in serum-free media or 10% FBS, and allowed to spread for 1-5 days. Following F-actin labeling using phalloidin, and immunolabeling of tubulin, alpha-smooth muscle actin ...[more]