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Capture and release of alkyne-derivatized glycerophospholipids using cobalt chemistry.

ABSTRACT: Alkyne-modified phospholipids can be unambiguously identified and differentiated from native species in complex mixtures by formation of dicobalthexacarbonyl complexes. This reaction is specific for alkynes and is unaffected by other glycerophospholipid-related moieties. Enrichment of cells with alkyne-derivatized fatty acids or glycerophospholipids followed by solid-phase sequestration and release is a promising new method for unequivocally monitoring individual glycerophospholipids following incorporation into cells. This technique also facilitates lipidomic analysis of substrates and products.

SUBMITTER: Milne SB

PROVIDER: S-EPMC2822082 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature

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Capture and release of alkyne-derivatized glycerophospholipids using cobalt chemistry.

Milne Stephen B SB Tallman Keri A KA Serwa Remigiusz R Rouzer Carol A CA Armstrong Michelle D MD Marnett Lawrence J LJ Lukehart Charles M CM Porter Ned A NA Brown H Alex HA

Nature chemical biology 20100124 3

Alkyne-modified phospholipids can be unambiguously identified and differentiated from native species in complex mixtures by formation of dicobalthexacarbonyl complexes. This reaction is specific for alkynes and is unaffected by other glycerophospholipid-related moieties. Enrichment of cells with alkyne-derivatized fatty acids or glycerophospholipids followed by solid-phase sequestration and release is a promising new method for unequivocally monitoring individual glycerophospholipids following i ...[more]

PMID: 20098428

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