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Temporal ChIP-on-Chip of RNA-Polymerase-II to detect novel gene activation events during photoreceptor maturation.


ABSTRACT:

SUBMITTER: Tummala P 

PROVIDER: S-EPMC2822553 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature

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Temporal ChIP-on-Chip of RNA-Polymerase-II to detect novel gene activation events during photoreceptor maturation.

Tummala Padmaja P   Mali Raghuveer S RS   Guzman Eduardo E   Zhang Xiao X   Mitton Kenneth P KP  

Molecular vision 20100217


<h4>Purpose</h4>During retinal development, post-mitotic neural progenitor cells must activate thousands of genes to complete synaptogenesis and terminal maturation. While many of these genes are known, others remain beyond the sensitivity of expression microarray analysis. Some of these elusive gene activation events can be detected by mapping changes in RNA polymerase-II (Pol-II) association around transcription start sites.<h4>Methods</h4>High-resolution (35 bp) chromatin immunoprecipitation  ...[more]

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