Ontology highlight
ABSTRACT:
SUBMITTER: Lukey MJ
PROVIDER: S-EPMC2823535 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
Lukey Michael J MJ Parkin Alison A Roessler Maxie M MM Murphy Bonnie J BJ Harmer Jeffrey J Palmer Tracy T Sargent Frank F Armstrong Fraser A FA
The Journal of biological chemistry 20091116 6
The enterobacterium Escherichia coli synthesizes two H(2) uptake enzymes, Hyd-1 and Hyd-2. We show using precise electrochemical kinetic measurements that the properties of Hyd-1 and Hyd-2 contrast strikingly, and may be individually optimized to function under distinct environmental conditions. Hyd-2 is well suited for fast and efficient catalysis in more reducing environments, to the extent that in vitro it behaves as a bidirectional hydrogenase. In contrast, Hyd-1 is active for H(2) oxidation ...[more]