SAMPLEX: automatic mapping of perturbed and unperturbed regions of proteins and complexes.

ABSTRACT:

Background

The activity of proteins within the cell is characterized by their motions, flexibility, interactions or even the particularly intriguing case of partially unfolded states. In the last two cases, a part of the protein is affected either by binding or unfolding and the detection of the respective perturbed and unperturbed region(s) is a fundamental part of the structural characterization of these states. This can be achieved by comparing experimental data of the same protein in two different states (bound/unbound, folded/unfolded). For instance, measurements of chemical shift perturbations (CSPs) from NMR 1H-15N HSQC experiments gives an excellent opportunity to discriminate both moieties.

Results

We describe an innovative, automatic and unbiased method to distingu

SUBMITTER: Krzeminski M 

PROVIDER: S-EPMC2823710 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature