Ontology highlight
ABSTRACT:
SUBMITTER: Voss NR
PROVIDER: S-EPMC2826578 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
Voss Neil R NR Lyumkis Dmitry D Cheng Anchi A Lau Pick-Wei PW Mulder Anke A Lander Gabriel C GC Brignole Edward J EJ Fellmann Denis D Irving Christopher C Jacovetty Erica L EL Leung Albert A Pulokas James J Quispe Joel D JD Winkler Hanspeter H Yoshioka Craig C Carragher Bridget B Potter Clinton S CS
Journal of structural biology 20091216 3
Structure determination of a novel macromolecular complex via single-particle electron microscopy depends upon overcoming the challenge of establishing a reliable 3-D reconstruction using only 2-D images. There are a variety of strategies that deal with this issue, but not all of them are readily accessible and straightforward to use. We have developed a "toolbox" of ab initio reconstruction techniques that provide several options for calculating 3-D volumes in an easily managed and tightly cont ...[more]