Ontology highlight
ABSTRACT:
SUBMITTER: Kim JW
PROVIDER: S-EPMC2827867 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 20100118 7-8
Protein quantification in a complex protein mixture presents a daunting task in biochemical analysis. Antibody-based immunoassays are traditional methods for protein quantification. However, there are issues associated with accuracy and specificity in these assays, especially when the changes are small (e.g., <2-fold). With recent developments in mass spectrometry, monitoring a selected peptide, thus protein, in a complex biological sample has become possible. In this study, we demonstrate a sim ...[more]