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Polyadenylation and degradation of incomplete RNA polymerase I transcripts in mammalian cells.


ABSTRACT: Most transcripts in growing cells are ribosomal RNA precursors (pre-rRNA). Here, we show that in mammals, aberrant pre-rRNA transcripts generated by RNA polymerase I (Pol I) are polyadenylated and accumulate markedly after treatment with low concentrations of actinomycin D (ActD), which blocks the synthesis of full-length rRNA. The poly(A) polymerase-associated domain-containing protein 5 is required for polyadenylation, whereas the exosome is partly responsible for the degradation of the short aberrant transcripts. Thus, polyadenylation functions in the quality control of Pol I transcription in metazoan cells. The impact of excessive aberrant RNAs on the degradation machinery is an unrecognized mechanism that might contribute to biological properties of ActD.

SUBMITTER: Shcherbik N 

PROVIDER: S-EPMC2828747 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature

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Polyadenylation and degradation of incomplete RNA polymerase I transcripts in mammalian cells.

Shcherbik Natalia N   Wang Minshi M   Lapik Yevgeniya R YR   Srivastava Leena L   Pestov Dimitri G DG  

EMBO reports 20100108 2


Most transcripts in growing cells are ribosomal RNA precursors (pre-rRNA). Here, we show that in mammals, aberrant pre-rRNA transcripts generated by RNA polymerase I (Pol I) are polyadenylated and accumulate markedly after treatment with low concentrations of actinomycin D (ActD), which blocks the synthesis of full-length rRNA. The poly(A) polymerase-associated domain-containing protein 5 is required for polyadenylation, whereas the exosome is partly responsible for the degradation of the short  ...[more]

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