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Intracellular delivery of a membrane-impermeable enzyme in active form using functionalized gold nanoparticles.

ABSTRACT: Gold nanoparticles were coated with a short peptide to promote intracellular delivery of membrane-impermeable proteins. Through microscopy and enzyme assays, we demonstrated the particles were able to transport functional enzymes into a variety of cell lines. Significantly, the transported proteins were able to escape from endosomes. Moreover, these particles showed no apparent cytotoxicity.

SUBMITTER: Ghosh P

PROVIDER: S-EPMC2830715 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature

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Intracellular delivery of a membrane-impermeable enzyme in active form using functionalized gold nanoparticles.

Ghosh Partha P Yang Xiaochao X Arvizo Rochelle R Zhu Zheng-Jiang ZJ Agasti Sarit S SS Mo Zhihong Z Rotello Vincent M VM

Journal of the American Chemical Society 20100301 8

Gold nanoparticles were coated with a short peptide to promote intracellular delivery of membrane-impermeable proteins. Through microscopy and enzyme assays, we demonstrated the particles were able to transport functional enzymes into a variety of cell lines. Significantly, the transported proteins were able to escape from endosomes. Moreover, these particles showed no apparent cytotoxicity. ...[more]

PMID: 20131834

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