Top-down proteomics reveals novel protein forms expressed in Methanosarcina acetivorans.
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ABSTRACT:
SUBMITTER: Ferguson JT
PROVIDER: S-EPMC2832193 | biostudies-literature | 2009 Sep
REPOSITORIES: biostudies-literature
Publications
Top-down proteomics reveals novel protein forms expressed in Methanosarcina acetivorans.
Ferguson Jonathan T JT Wenger Craig D CD Metcalf William W WW Kelleher Neil L NL
Journal of the American Society for Mass Spectrometry 20090606 9
Using both automated nanospray and online liquid chromatography mass spectrometry LC-MS strategies, 99 proteins have been newly identified by top-down tandem mass spectrometry (MS/MS) in Methanosarcina acetivorans, the methanogen with the largest known genome [5.7 mega base pairs (Mb)] for an Archaeon. Because top-down MS/MS was used, 15 proteins were detected with mispredicted start sites along with an additional five from small open reading frames (SORFs). Beyond characterization of these more ...[more]
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