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PilF Polymorphism-based PCR to distinguish vibrio vulnificus strains potentially dangerous to public health.

ABSTRACT: Vibrio vulnificus is a heterogeneous species that comprises strains virulent and avirulent for humans and fish, and it is grouped into three biotypes. In this report, we describe a PCR-based methodology that allows both the species identification and discrimination of those isolates that could be considered dangerous to public health. Discrimination is based on the amplification of a variable region located within the gene pilF, which seems to be associated with potential human pathogenicity, regardless of the biotype of the strain.

SUBMITTER: Roig FJ

PROVIDER: S-EPMC2832365 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature

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pilF Polymorphism-based PCR to distinguish vibrio vulnificus strains potentially dangerous to public health.

Roig Francisco J FJ Sanjuán Eva E Llorens Amparo A Amaro Carmen C

Applied and environmental microbiology 20091228 5

Vibrio vulnificus is a heterogeneous species that comprises strains virulent and avirulent for humans and fish, and it is grouped into three biotypes. In this report, we describe a PCR-based methodology that allows both the species identification and discrimination of those isolates that could be considered dangerous to public health. Discrimination is based on the amplification of a variable region located within the gene pilF, which seems to be associated with potential human pathogenicity, re ...[more]

PMID: 20038687

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