Systematic domain deletion analysis of the major sporulation kinase in Bacillus subtilis.
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ABSTRACT:
SUBMITTER: Eswaramoorthy P
PROVIDER: S-EPMC2832533 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
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Systematic domain deletion analysis of the major sporulation kinase in Bacillus subtilis.
Eswaramoorthy Prahathees P Fujita Masaya M
Journal of bacteriology 20100115 6
To characterize the role of the three PAS domains in KinA, the major sporulation kinase in Bacillus subtilis, we constructed a series of systematic PAS domain deletion mutants and analyzed their activities using an IPTG (isopropyl-beta-d-thiogalactopyranoside)-inducible artificial sporulation induction system, which we have developed recently. The results showed that any one of the three PAS domains is sufficient to maintain the kinase activity and trigger sporulation, if not fully then at least ...[more]
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