Ontology highlight
ABSTRACT:
SUBMITTER: Soulimane T
PROVIDER: S-EPMC2833055 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
Soulimane Tewfik T O'Kane Sarah R SR Kolaj Olga O
Acta crystallographica. Section F, Structural biology and crystallization communications 20100227 Pt 3
The oxygenase HpaB is a component of the 4-hydroxyphenylacetate 3-monooxygenase enzyme that is responsible for the hydroxylation of 4-hydroxyphenylacetate. It utilizes molecular oxygen and a reduced flavin, which is provided by HpaC, the second component of the enzyme. While isolating integral membrane respiratory complexes from Thermus thermophilus, microcrystals of HpaB were formed. Further purification of the enzyme was achieved by repetitive crystallization. Subsequently, well shaped single ...[more]