Conversion of the allosteric transition of GroEL from concerted to sequential by the single mutation Asp-155 -> Ala.

ABSTRACT: The reaction cycle of the double-ring chaperonin GroEL is driven by ATP binding that takes place with positive cooperativity within each seven-membered ring and negative cooperativity between rings. The positive cooperativity within rings is due to ATP binding-induced conformational changes that are fully concerted. Herein, it is shown that the mutation Asp-155 --> Ala leads to an ATP-induced break in intra-ring and inter-ring symmetry. Electron microscopy analysis of single-ring GroEL particles containing the Asp-155 --> Ala mutation shows that the break in intra-ring symmetry is due to stabilization of allosteric intermediates such as one in which three subunits have switched their conformation while the other four have not. Our results show that eliminating an intra-subunit interaction

SUBMITTER: Danziger O 

PROVIDER: S-EPMC283501 | biostudies-literature | 2003 Nov

REPOSITORIES: biostudies-literature