A pilot study of high-throughput, sequence-based mutational profiling of primary human acute myeloid leukemia cell genomes.
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ABSTRACT:
SUBMITTER: Ley TJ
PROVIDER: S-EPMC283582 | biostudies-literature | 2003 Nov
REPOSITORIES: biostudies-literature
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A pilot study of high-throughput, sequence-based mutational profiling of primary human acute myeloid leukemia cell genomes.
Ley Timothy J TJ Minx Patrick J PJ Walter Matthew J MJ Ries Rhonda E RE Sun Hui H McLellan Michael M DiPersio John F JF Link Daniel C DC Tomasson Michael H MH Graubert Timothy A TA McLeod Howard H Khoury Hanna H Watson Mark M Shannon William W Trinkaus Kathryn K Heath Sharon S Vardiman James W JW Caligiuri Michael A MA Bloomfield Clara D CD Milbrandt Jeffrey D JD Mardis Elaine R ER Wilson Richard K RK
Proceedings of the National Academy of Sciences of the United States of America 20031112 24
In this pilot study, we used primary human acute myeloid leukemia (AML) cell genomes as templates for exonic PCR amplification, followed by high-throughput resequencing, analyzing approximately 7 million base pairs of DNA from 140 AML samples and 48 controls. We identified six previously described, and seven previously undescribed sequence changes that may be relevant for AML pathogenesis. Because the sequencing templates were generated from primary AML cells, the technique favors the detection ...[more]
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