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Genes required for mitotic spindle assembly in Drosophila S2 cells.

ABSTRACT: The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.

SUBMITTER: Goshima G

PROVIDER: S-EPMC2837481 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature

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Genes required for mitotic spindle assembly in Drosophila S2 cells.

Goshima Gohta G Wollman Roy R Goodwin Sarah S SS Zhang Nan N Scholey Jonathan M JM Vale Ronald D RD Stuurman Nico N

Science (New York, N.Y.) 20070405 5823

The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes a ...[more]

PMID: 17412918

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