Ontology highlight
ABSTRACT:
SUBMITTER: Krasnoperov LN
PROVIDER: S-EPMC2839868 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
Krasnoperov Lev N LN Marras Salvatore A E SA Kozlov Maxim M Wirpsza Laura L Mustaev Arkady A
Bioconjugate chemistry 20100201 2
Novel amino-reactive derivatives of lanthanide-based luminescent labels of enhanced brightness and metal retention were synthesized and used for the detection of cDNA oligonucleotides by molecular beacons. Time-resolved acquisition of the luminescent signal that occurs upon hybridization of the probe to the target enabled the avoidance of short-lived background fluorescence, markedly enhancing the sensitivity of detection, which was less than 1 pM. This value is about 50 to 100 times more sensit ...[more]