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Glycopolydiacetylene nanoparticles as a chromatic biosensor to detect Shiga-like toxin producing Escherichia coli O157:H7.


ABSTRACT: Shiga toxin-producing Escherichia coli organisms (STEC) were detected by Gal-alpha1,4-Gal glycopolydiacetylene (GPDA) nanoparticles through the selective binding between Shiga toxin and GPDA nanoparticles. The binding produced a colorimetric change in the absorption wavelength of the GPDA nanoparticles. This method provides a highly selective, rapid, sensitive, and quantitative approach for the detection of STEC.

SUBMITTER: Nagy JO 

PROVIDER: S-EPMC2839895 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature

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Glycopolydiacetylene nanoparticles as a chromatic biosensor to detect Shiga-like toxin producing Escherichia coli O157:H7.

Nagy Jon O JO   Zhang Yalong Y   Yi Wen W   Liu Xianwei X   Motari Edwin E   Song Jing Catherine JC   Lejeune Jeffrey T JT   Wang Peng George PG  

Bioorganic & medicinal chemistry letters 20071121 2


Shiga toxin-producing Escherichia coli organisms (STEC) were detected by Gal-alpha1,4-Gal glycopolydiacetylene (GPDA) nanoparticles through the selective binding between Shiga toxin and GPDA nanoparticles. The binding produced a colorimetric change in the absorption wavelength of the GPDA nanoparticles. This method provides a highly selective, rapid, sensitive, and quantitative approach for the detection of STEC. ...[more]

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