Ontology highlight
ABSTRACT:
SUBMITTER: Andreev OA
PROVIDER: S-EPMC2840156 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
Andreev Oleg A OA Karabadzhak Alexander G AG Weerakkody Dhammika D Andreev Gregory O GO Engelman Donald M DM Reshetnyak Yana K YK
Proceedings of the National Academy of Sciences of the United States of America 20100216 9
What are the molecular events that occur when a peptide inserts across a membrane or exits from it? Using the pH-triggered insertion of the pH low insertion peptide to enable kinetic analysis, we show that insertion occurs in several steps, with rapid (0.1 sec) interfacial helix formation, followed by a much slower (100 sec) insertion pathway to give a transmembrane helix. The reverse process of unfolding and peptide exit from the bilayer core, which can be induced by a rapid rise of the pH from ...[more]