Compartmentalization by directional gene expression.
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ABSTRACT:
SUBMITTER: Daube SS
PROVIDER: S-EPMC2840370 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
Publications
Compartmentalization by directional gene expression.
Daube Shirley S SS Bracha Dan D Buxboim Amnon A Bar-Ziv Roy H RH
Proceedings of the National Academy of Sciences of the United States of America 20100201 7
The coalescence of basic biochemical reactions into compartments is a major hallmark of a living cell. Using surface-bound DNA and a transcription reaction, we investigate the conditions for boundary-free compartmentalization. The DNA self-organizes into a dense and ordered phase with coding sequences aligned at well-defined distances and orientation relative to the surface, imposing directionality on transcription. Unique to the surface in comparison to dilute homogeneous DNA solution, the reac ...[more]
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