Ontology highlight
ABSTRACT:
SUBMITTER: Baxter NJ
PROVIDER: S-EPMC2842025 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
Baxter Nicola J NJ Bowler Matthew W MW Alizadeh Tooba T Cliff Matthew J MJ Hounslow Andrea M AM Wu Bin B Berkowitz David B DB Williams Nicholas H NH Blackburn G Michael GM Waltho Jonathan P JP
Proceedings of the National Academy of Sciences of the United States of America 20100217 10
Prior evidence supporting the direct observation of phosphorane intermediates in enzymatic phosphoryl transfer reactions was based on the interpretation of electron density corresponding to trigonal species bridging the donor and acceptor atoms. Close examination of the crystalline state of beta-phosphoglucomutase, the archetypal phosphorane intermediate-containing enzyme, reveals that the trigonal species is not PO-3 , but is MgF-3 (trifluoromagnesate). Although MgF-3 complexes are transition s ...[more]