A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation.
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ABSTRACT:
SUBMITTER: Schwarz F
PROVIDER: S-EPMC2842479 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
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A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation.
Schwarz Flavio F Huang Wei W Li Cishan C Schulz Benjamin L BL Lizak Christian C Palumbo Alessandro A Numao Shin S Neri Dario D Aebi Markus M Wang Lai-Xi LX
Nature chemical biology 20100228 4
We describe a new method for producing homogeneous eukaryotic N-glycoproteins. The method involves the engineering and functional transfer of the Campylobacter jejuni glycosylation machinery in Escherichia coli to express glycosylated proteins with the key GlcNAc-Asn linkage. The bacterial glycans were then trimmed and remodeled in vitro by enzymatic transglycosylation to fulfill a eukaryotic N-glycosylation. It provides a potentially general platform for producing eukaryotic N-glycoproteins. ...[more]
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