Pathways by which reconstituted high-density lipoprotein mobilizes free cholesterol from whole body and from macrophages.

ABSTRACT:

Objective

Reconstituted high-density lipoprotein (rHDL) is of interest as a potential novel therapy for atherosclerosis because of its ability to promote free cholesterol (FC) mobilization after intravenous administration. We performed studies to identify the underlying molecular mechanisms by which rHDL promote FC mobilization from whole body in vivo and macrophages in vitro.

Methods and results

Wild-type (WT), SR-BI knockout (KO), ABCA1 KO, and ABCG1 KO mice received either rHDL or phosphate-buffered saline intravenously. Blood was drawn before and at several time points after injection for apolipoprotein A-I, phosphatidylcholine, and FC measurement. In WT mice, serum FC peaked at 20 minutes and rapidly returned toward baseline levels by 24 hours. Unexpectedly, ABCA1 KO an

SUBMITTER: Cuchel M 

PROVIDER: S-EPMC2842952 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature