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Cell-penetrating peptides with intracellular actin-remodeling activity in malignant fibroblasts.

ABSTRACT: Cell-penetrating peptides can cross cell membranes and are commonly seen as biologically inert molecules. However, we found that some cell-penetrating peptides could remodel actin cytoskeleton in oncogene-transformed NIH3T3/EWS-Fli cells. These cells have profound actin disorganization related to their tumoral transformation. These arginine- and/or tryptophan-rich peptides could cross cell membrane and induce stress fiber formation in these malignant cells, whereas they had no perceptible effect in non-tumoral fibroblasts. In addition, motility (migration speed, random motility coefficient, wound healing) of the tumor cells could be decreased by the cell-permeant peptides. Although the peptides differently influenced actin polymerization in vitro, they could directly bind monomeric actin as determined by NMR and calorimetry studies. Therefore, cell-penetrating peptides might interact with intracellular protein partners, such as actin. In addition, the fact that they could reverse the tumoral phenotype is of interest for therapeutic purposes.

SUBMITTER: Delaroche D

PROVIDER: S-EPMC2844216 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature

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Cell-penetrating peptides with intracellular actin-remodeling activity in malignant fibroblasts.

Delaroche Diane D Cantrelle François-Xavier FX Subra Frédéric F Van Heijenoort Carine C Guittet Eric E Jiao Chen-Yu CY Blanchoin Laurent L Chassaing Gérard G Lavielle Solange S Auclair Christian C Sagan Sandrine S

The Journal of biological chemistry 20091227 10

Cell-penetrating peptides can cross cell membranes and are commonly seen as biologically inert molecules. However, we found that some cell-penetrating peptides could remodel actin cytoskeleton in oncogene-transformed NIH3T3/EWS-Fli cells. These cells have profound actin disorganization related to their tumoral transformation. These arginine- and/or tryptophan-rich peptides could cross cell membrane and induce stress fiber formation in these malignant cells, whereas they had no perceptible effect ...[more]

PMID: 20037163

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Dataset Information

Cell-penetrating peptides with intracellular actin-remodeling activity in malignant fibroblasts.

Publications

Cell-penetrating peptides with intracellular actin-remodeling activity in malignant fibroblasts.

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