Ontology highlight
ABSTRACT:
SUBMITTER: Lee HR
PROVIDER: S-EPMC2845987 | biostudies-literature | 2009 Mar
REPOSITORIES: biostudies-literature
Lee Harold R HR Wang Mina M Konigsberg William W
Biochemistry 20090301 10
Two divalent metal ions are required for nucleotide incorporation by DNA polymerases. Here we use the bacteriophage RB69 DNA polymerase (RB69 pol) and the metal ion exchange-inert nucleotide analogue rhodium(III) deoxythymidine triphosphate (Rh.dTTP) to investigate the requirements of metal binding to the "A" site and to the "B" site, independently. We show that while binding of a metal ion to the A site is required for the nucleotidyl transfer reaction to occur, this metal binding is insufficie ...[more]