Ontology highlight
ABSTRACT:
SUBMITTER: Grizot S
PROVIDER: S-EPMC2847234 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Nucleic acids research 20091221 6
Homing endonucleases have become valuable tools for genome engineering. Their sequence recognition repertoires can be expanded by modifying their specificities or by creating chimeric proteins through domain swapping between two subdomains of different homing endonucleases. Here, we show that these two approaches can be combined to create engineered meganucleases with new specificities. We demonstrate the modularity of the chimeric DmoCre meganuclease previously described, by successfully assemb ...[more]