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DNA stretching in the nucleosome facilitates alkylation by an intercalating antitumour agent.

ABSTRACT: DNA stretching in the nucleosome core can cause dramatic structural distortions, which may influence compaction and factor recognition in chromatin. We find that the base pair unstacking arising from stretching-induced extreme minor groove kinking near the nucleosome centre creates a hot spot for intercalation and alkylation by a novel anticancer compound. This may have far reaching implications for how chromatin structure can influence binding of intercalator species and indicates potential for the development of site selective DNA-binding agents that target unique conformational features of the nucleosome.

SUBMITTER: Davey GE

PROVIDER: S-EPMC2847236 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature

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DNA stretching in the nucleosome facilitates alkylation by an intercalating antitumour agent.

Davey Gabriela E GE Wu Bin B Dong Yuancai Y Surana Uttam U Davey Curt A CA

Nucleic acids research 20091221 6

DNA stretching in the nucleosome core can cause dramatic structural distortions, which may influence compaction and factor recognition in chromatin. We find that the base pair unstacking arising from stretching-induced extreme minor groove kinking near the nucleosome centre creates a hot spot for intercalation and alkylation by a novel anticancer compound. This may have far reaching implications for how chromatin structure can influence binding of intercalator species and indicates potential for ...[more]

PMID: 20026584

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