Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data.
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ABSTRACT:
SUBMITTER: Marullo M
PROVIDER: S-EPMC2847721 | biostudies-literature | 2010
REPOSITORIES: biostudies-literature
Publications
Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data.
Marullo Manuela M Zuccato Chiara C Mariotti Caterina C Lahiri Nayana N Tabrizi Sarah J SJ Di Donato Stefano S Cattaneo Elena E
Genome biology 20100128 1
We describe a novel strategy for mRNA normalization in quantitative real-time PCR that is based on expressed Alu repeat amplification as a measure for the mRNA fraction. We show that expressed Alu repeat amplification is a fast, accurate normalization tool that can be successfully used for quantification of selected mRNA in the human transcriptome. This result is particularly important for clinical diagnosis and biomarker validation studies based on mRNA detection in human blood. ...[more]
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