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Acoustophoretic synchronization of mammalian cells in microchannels.

ABSTRACT: We report the first use of ultrasonic standing waves to achieve cell cycle phase synchronization in mammalian cells in a high-throughput and reagent-free manner. The acoustophoretic cell synchronization (ACS) device utilizes volume-dependent acoustic radiation force within a microchannel to selectively purify target cells of desired phase from an asynchronous mixture based on cell cycle-dependent fluctuations in size. We show that ultrasonic separation allows for gentle, scalable, and label-free synchronization with high G(1) phase synchrony (approximately 84%) and throughput (3 x 10(6) cells/h per microchannel).

SUBMITTER: Thevoz P

PROVIDER: S-EPMC2848285 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature

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Acoustophoretic synchronization of mammalian cells in microchannels.

Thévoz Patrick P Adams Jonathan D JD Shea Herbert H Bruus Henrik H Soh H Tom HT

Analytical chemistry 20100401 7

We report the first use of ultrasonic standing waves to achieve cell cycle phase synchronization in mammalian cells in a high-throughput and reagent-free manner. The acoustophoretic cell synchronization (ACS) device utilizes volume-dependent acoustic radiation force within a microchannel to selectively purify target cells of desired phase from an asynchronous mixture based on cell cycle-dependent fluctuations in size. We show that ultrasonic separation allows for gentle, scalable, and label-free ...[more]

PMID: 20199060

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