New efficient recombinant expression system to engineer Candida antarctica lipase B.
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ABSTRACT:
SUBMITTER: Emond S
PROVIDER: S-EPMC2849202 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
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New efficient recombinant expression system to engineer Candida antarctica lipase B.
Emond Stéphane S Montanier Cédric C Nicaud Jean-Marc JM Marty Alain A Monsan Pierre P André Isabelle I Remaud-Siméon Magali M
Applied and environmental microbiology 20100219 8
Here, we report the use of Yarrowia lipolytica as a versatile expression host for developing protein engineering approaches to modify the properties of Candida antarctica lipase B. A reliable screening protocol was defined and validated using a saturation mutagenesis library, yielding mutants displaying higher catalytic efficiencies than the wild-type enzyme. ...[more]
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