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Characterization of the Aspergillus nidulans monodictyphenone gene cluster.

ABSTRACT: Deletion of cclA, a component of the COMPASS complex of Aspergillus nidulans, results in the production of monodictyphenone and emodin derivatives. Through a set of targeted deletions in a cclA deletion strain, we have identified the genes required for monodictyphenone and emodin analog biosynthesis. Identification of an intermediate, endocrocin, from an mdpHDelta strain suggests that mdpH might encode a decarboxylase. Furthermore, by replacing the promoter of mdpA (a putative aflJ homolog) and mdpE (a putative aflR homolog) with the inducible alcA promoter, we have confirmed that MdpA functions as a coactivator. We propose a biosynthetic pathway for monodictyphenone and emodin derivatives based on bioinformatic analysis and characterization of biosynthetic intermediates.

SUBMITTER: Chiang YM

PROVIDER: S-EPMC2849234 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature

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Characterization of the Aspergillus nidulans monodictyphenone gene cluster.

Chiang Yi-Ming YM Szewczyk Edyta E Davidson Ashley D AD Entwistle Ruth R Keller Nancy P NP Wang Clay C C CC Oakley Berl R BR

Applied and environmental microbiology 20100205 7

Deletion of cclA, a component of the COMPASS complex of Aspergillus nidulans, results in the production of monodictyphenone and emodin derivatives. Through a set of targeted deletions in a cclA deletion strain, we have identified the genes required for monodictyphenone and emodin analog biosynthesis. Identification of an intermediate, endocrocin, from an mdpHDelta strain suggests that mdpH might encode a decarboxylase. Furthermore, by replacing the promoter of mdpA (a putative aflJ homolog) and ...[more]

PMID: 20139316

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