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Rapid and reliable single nucleotide polymorphism-based differentiation of Brucella live vaccine strains from field strains.

ABSTRACT: The reliable differentiation of live Brucella vaccine strains from field isolates is an important element in brucellosis control programs. We describe the design, validation, and implementation of a novel single nucleotide polymorphism (SNP)-based typing platform that offers a rapid, reliable, and robust tool to achieve this with improved diagnostic accuracy compared to existing molecular tests. Furthermore, the assays described are designed such that they supplement, and can be run as an intrinsic part of, a previously described assay identifying Brucella isolates to the species level (K. K. Gopaul, C. J. Smith, M. S. Koylass, and A. M. Whatmore, BMC Microbiol. 8:86), giving a comprehensive molecular typing platform.

SUBMITTER: Gopaul KK

PROVIDER: S-EPMC2849582 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature

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Rapid and reliable single nucleotide polymorphism-based differentiation of Brucella live vaccine strains from field strains.

Gopaul Krishna K KK Sells Jessica J Bricker Betsy J BJ Crasta Oswald R OR Whatmore Adrian M AM

Journal of clinical microbiology 20100224 4

The reliable differentiation of live Brucella vaccine strains from field isolates is an important element in brucellosis control programs. We describe the design, validation, and implementation of a novel single nucleotide polymorphism (SNP)-based typing platform that offers a rapid, reliable, and robust tool to achieve this with improved diagnostic accuracy compared to existing molecular tests. Furthermore, the assays described are designed such that they supplement, and can be run as an intrin ...[more]

PMID: 20181906

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