Dataset Information

Functional and evolutionary insights from the genomes of three parasitoid Nasonia species.

ABSTRACT: We report here genome sequences and comparative analyses of three closely related parasitoid wasps: Nasonia vitripennis, N. giraulti, and N. longicornis. Parasitoids are important regulators of arthropod populations, including major agricultural pests and disease vectors, and Nasonia is an emerging genetic model, particularly for evolutionary and developmental genetics. Key findings include the identification of a functional DNA methylation tool kit; hymenopteran-specific genes including diverse venoms; lateral gene transfers among Pox viruses, Wolbachia, and Nasonia; and the rapid evolution of genes involved in nuclear-mitochondrial interactions that are implicated in speciation. Newly developed genome resources advance Nasonia for genetic research, accelerate mapping and cloning of quantitative trait loci, and will ultimately provide tools and knowledge for further increasing the utility of parasitoids as pest insect-control agents.

SUBMITTER: Werren JH

PROVIDER: S-EPMC2849982 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Functional and evolutionary insights from the genomes of three parasitoid Nasonia species.

Werren John H JH Richards Stephen S Desjardins Christopher A CA Niehuis Oliver O Gadau Jürgen J Colbourne John K JK Werren John H JH Richards Stephen S Desjardins Christopher A CA Niehuis Oliver O Gadau Jürgen J Colbourne John K JK Beukeboom Leo W LW Desplan Claude C Elsik Christine G CG Grimmelikhuijzen Cornelis J P CJ Kitts Paul P Lynch Jeremy A JA Murphy Terence T Oliveira Deodoro C S G DC Smith Christopher D CD van de Zande Louis L Worley Kim C KC Zdobnov Evgeny M EM Aerts Maarten M Albert Stefan S Anaya Victor H VH Anzola Juan M JM Barchuk Angel R AR Behura Susanta K SK Bera Agata N AN Berenbaum May R MR Bertossa Rinaldo C RC Bitondi Márcia M G MM Bordenstein Seth R SR Bork Peer P Bornberg-Bauer Erich E Brunain Marleen M Cazzamali Giuseppe G Chaboub Lesley L Chacko Joseph J Chavez Dean D Childers Christopher P CP Choi Jeong-Hyeon JH Clark Michael E ME Claudianos Charles C Clinton Rochelle A RA Cree Andrew G AG Cristino Alexandre S AS Dang Phat M PM Darby Alistair C AC de Graaf Dirk C DC Devreese Bart B Dinh Huyen H HH Edwards Rachel R Elango Navin N Elhaik Eran E Ermolaeva Olga O Evans Jay D JD Foret Sylvain S Fowler Gerald R GR Gerlach Daniel D Gibson Joshua D JD Gilbert Donald G DG Graur Dan D Gründer Stefan S Hagen Darren E DE Han Yi Y Hauser Frank F Hultmark Da D Hunter Henry C HC Hurst Gregory D D GD Jhangian Shalini N SN Jiang Huaiyang H Johnson Reed M RM Jones Andrew K AK Junier Thomas T Kadowaki Tatsuhiko T Kamping Albert A Kapustin Yuri Y Kechavarzi Bobak B Kim Jaebum J Kim Jay J Kiryutin Boris B Koevoets Tosca T Kovar Christie L CL Kriventseva Evgenia V EV Kucharski Robert R Lee Heewook H Lee Sandra L SL Lees Kristin K Lewis Lora R LR Loehlin David W DW Logsdon John M JM Lopez Jacqueline A JA Lozado Ryan J RJ Maglott Donna D Maleszka Ryszard R Mayampurath Anoop A Mazur Danielle J DJ McClure Marcella A MA Moore Andrew D AD Morgan Margaret B MB Muller Jean J Munoz-Torres Monica C MC Muzny Donna M DM Nazareth Lynne V LV Neupert Susanne S Nguyen Ngoc B NB Nunes Francis M F FM Oakeshott John G JG Okwuonu Geoffrey O GO Pannebakker Bart A BA Pejaver Vikas R VR Peng Zuogang Z Pratt Stephen C SC Predel Reinhard R Pu Ling-Ling LL Ranson Hilary H Raychoudhury Rhitoban R Rechtsteiner Andreas A Reese Justin T JT Reid Jeffrey G JG Riddle Megan M Robertson Hugh M HM Romero-Severson Jeanne J Rosenberg Miriam M Sackton Timothy B TB Sattelle David B DB Schlüns Helge H Schmitt Thomas T Schneider Martina M Schüler Andreas A Schurko Andrew M AM Shuker David M DM Simões Zilá L P ZL Sinha Saurabh S Smith Zachary Z Solovyev Victor V Souvorov Alexandre A Springauf Andreas A Stafflinger Elisabeth E Stage Deborah E DE Stanke Mario M Tanaka Yoshiaki Y Telschow Arndt A Trent Carol C Vattathil Selina S Verhulst Eveline C EC Viljakainen Lumi L Wanner Kevin W KW Waterhouse Robert M RM Whitfield James B JB Wilkes Timothy E TE Williamson Michael M Willis Judith H JH Wolschin Florian F Wyder Stefan S Yamada Takuji T Yi Soojin V SV Zecher Courtney N CN Zhang Lan L Gibbs Richard A RA

Science (New York, N.Y.) 20100101 5963

We report here genome sequences and comparative analyses of three closely related parasitoid wasps: Nasonia vitripennis, N. giraulti, and N. longicornis. Parasitoids are important regulators of arthropod populations, including major agricultural pests and disease vectors, and Nasonia is an emerging genetic model, particularly for evolutionary and developmental genetics. Key findings include the identification of a functional DNA methylation tool kit; hymenopteran-specific genes including diverse ...[more]

PMID: 20075255

Similar Datasets

Project description:Parasitoid wasps are a diverse group of parasitoid insects that play a crucial role in biological control and integrated pest management programs due to their wide range of host species and complex behaviors. Previous studies have reported that select RNA viruses in parasitoid wasps can affect either the host-parasitoid wasp or the pest host parasitized by the wasps. Therefore, a study of the dynamics of RNA viruses in parasitoid wasps is essential for artificial breeding programs and improving their parasitic capabilities. In this study, we detected the presence of nine novel and three known RNA viruses with complete genomes in three parasitoid wasp species: Diachasmimorpha longicaudata, Fopius arisanus, and Spalangia endius. These wasps are parasitoids that can parasitel tephritid flies, one of the most important pest groups of fruits and vegetables. In D. longicaudata, the small RNAs derived from the viruses exhibited distinct peaks at 22 nt and were symmetrically distributed across the viral genome. These findings indicate that these viruses can activate the host's RNAi immune response. PCR detection further revealed that DlNaLV efficiently infects female individuals of D. longicaudata with 100% efficiency, and ZcNLV is capable of infecting both D. longicaudata and its host, Zeugodacus cucurbitae. Our findings contribute to a better understanding of RNA viruses in parasitoid wasps and lay the foundation for future research on the triple-level trophic relationships between tephritid flies, parasitoid wasps, and RNA viruses.IMPORTANCEParasitoid wasp populations have developed persistent beneficial symbiotic relationships with several viruses through repeated evolution. However, there have been limited reports on RNA viruses in parasitoid wasps of tephritid flies, a significant pest group affecting fruits and vegetables. This study explores the diversity of RNA viruses in three parasitoid wasps of tephritid flies and highlights the potential biological significance of specific viruses in Diachasmimorpha longicaudata. These findings have important implications for the development of sustainable pest management strategies and the enhancement of artificial rearing techniques for parasitoid wasps.

Project description:BACKGROUND: The Ralstonia solanacearum species complex includes thousands of strains pathogenic to an unusually wide range of plant species. These globally dispersed and heterogeneous strains cause bacterial wilt diseases, which have major socio-economic impacts. Pathogenicity is an ancestral trait in R. solanacearum and strains with high genetic variation can be subdivided into four phylotypes, correlating to isolates from Asia (phylotype I), the Americas (phylotype IIA and IIB), Africa (phylotype III) and Indonesia (phylotype IV). Comparison of genome sequences strains representative of this phylogenetic diversity can help determine which traits allow this bacterium to be such a pathogen of so many different plant species and how the bacteria survive in many different habitats. RESULTS: The genomes of three tomato bacterial wilt pathogens, CFBP2957 (phy. IIA), CMR15 (phy. III) and PSI07 (phy. IV) were sequenced and manually annotated. These genomes were compared with those of three previously sequenced R. solanacearum strains: GMI1000 (tomato, phy. I), IPO1609 (potato, phy. IIB), and Molk2 (banana, phy. IIB). The major genomic features (size, G+C content, number of genes) were conserved across all of the six sequenced strains. Despite relatively high genetic distances (calculated from average nucleotide identity) and many genomic rearrangements, more than 60% of the genes of the megaplasmid and 70% of those on the chromosome are syntenic. The three new genomic sequences revealed the presence of several previously unknown traits, probably acquired by horizontal transfers, within the genomes of R. solanacearum, including a type IV secretion system, a rhi-type anti-mitotic toxin and two small plasmids. Genes involved in virulence appear to be evolving at a faster rate than the genome as a whole. CONCLUSIONS: Comparative analysis of genome sequences and gene content confirmed the differentiation of R. solanacearum species complex strains into four phylotypes. Genetic distances between strains, in conjunction with CGH analysis of a larger set of strains, revealed differences great enough to consider reclassification of the R. solanacearum species complex into three species. The data are still too fragmentary to link genomic classification and phenotypes, but these new genome sequences identify a pan-genome more representative of the diversity in the R. solanancearum species complex.

Project description:Linking behavioural phenotypes to their underlying genotypes is crucial for uncovering the mechanisms that underpin behaviour and for understanding the origins and maintenance of genetic variation in behaviour. Recently, interest has begun to focus on the transcriptome as a route for identifying genes and gene pathways associated with behaviour. For many behavioural traits studied at the phenotypic level, we have little or no idea of where to start searching for "candidate" genes: the transcriptome provides such a starting point. Here we consider transcriptomic changes associated with oviposition in the parasitoid wasp Nasonia vitripennis. Oviposition is a key behaviour for parasitoids, as females are faced with a variety of decisions that will impact offspring fitness. These include choosing between hosts of differing quality, as well as making decisions regarding clutch size and offspring sex ratio. We compared the whole-body transcriptomes of resting or ovipositing female Nasonia using a "DeepSAGE" gene expression approach on the Illumina sequencing platform. We identified 332 tags that were significantly differentially expressed between the two treatments, with 77% of the changes associated with greater expression in resting females. Oviposition therefore appears to focus gene expression away from a number of physiological processes, with gene ontologies suggesting that aspects of metabolism may be down-regulated during egg-laying. Nine of the most abundant differentially expressed tags showed greater expression in ovipositing females though, including the genes purity-of-essence (associated with behavioural phenotypes in Drosophila) and glucose dehydrogenase (GLD). The GLD protein has been implicated in sperm storage and release in Drosophila and so provides a possible candidate for the control of sex allocation by female Nasonia during oviposition. Oviposition in Nasonia therefore clearly modifies the transcriptome, providing a starting point for the genetic dissection of oviposition.

Project description:Homologous meiotic recombination occurs in most sexually reproducing organisms, yet its evolutionary advantages are elusive. Previous research explored recombination in the honeybee, a eusocial hymenopteran with an exceptionally high genome-wide recombination rate. A comparable study in a non-social member of the Hymenoptera that would disentangle the impact of sociality from Hymenoptera-specific features such as haplodiploidy on the evolution of the high genome-wide recombination rate in social Hymenoptera is missing. Utilizing single-nucleotide polymorphisms (SNPs) between two Nasonia parasitoid wasp genomes, we developed a SNP genotyping microarray to infer a high-density linkage map for Nasonia. The map comprises 1,255 markers with an average distance of 0.3 cM. The mapped markers enabled us to arrange 265 scaffolds of the Nasonia genome assembly 1.0 on the linkage map, representing 63.6% of the assembled N. vitripennis genome. We estimated a genome-wide recombination rate of 1.4-1.5 cM/Mb for Nasonia, which is less than one tenth of the rate reported for the honeybee. The local recombination rate in Nasonia is positively correlated with the distance to the center of the linkage groups, GC content, and the proportion of simple repeats. In contrast to the honeybee genome, gene density in the parasitoid wasp genome is positively associated with the recombination rate; regions of low recombination are characterized by fewer genes with larger introns and by a greater distance between genes. Finally, we found that genes in regions of the genome with a low recombination frequency tend to have a higher ratio of non-synonymous to synonymous substitutions, likely due to the accumulation of slightly deleterious non-synonymous substitutions. These findings are consistent with the hypothesis that recombination reduces interference between linked sites and thereby facilitates adaptive evolution and the purging of deleterious mutations. Our results imply that the genomes of haplodiploid and of diploid higher eukaryotes do not differ systematically in their recombination rates and associated parameters.

Project description:Background The generalist dipteran pupal parasitoid Nasonia vitripennis injects 79 venom peptides into the host before egg laying. This venom induces several important changes in the host, including developmental arrest, immunosuppression, and alterations to normal metabolism. It is hoped that diverse and potent bioactivities of N. vitripennis venom provide an opportunity for the design of novel acting drugs. However, currently very little is known about the individual functions of N. vitripennis venom peptides and less than half can be bioinformatically annotated. The paucity of annotation information complicates the design of studies that seek to better understand the potential mechanisms underlying the envenomation response. Although the RNA interference system of N. vitripennis provides an opportunity to functionally characterise venom encoding genes, with 79 candidates this represents a daunting task. For this reason we were interested in determining the expression levels of venom encoding genes in the venom gland, such that this information could be used to rank candidate venoms. To do this we carried out deep sequencing of the transcriptome of the venom gland and neighbouring ovary tissue and used RNA-seq to measure expression from the 79 venom encoding genes. The generation of a specific venom gland transcriptome dataset also provides further opportunities to investigate novel features of this highly specialised organ. Results High throughput sequencing and RNA-seq revealed that the highest expressed venom encoding gene in the venom gland was a serine protease called Nasvi2EG007167, which has previously been implicated in the apoptotic activity of N. vitripennis venom. As expected the RNA-seq confirmed that the N. vitripennis venom encoding genes are almost exclusively expressed in the venom gland relative to the neighbouring ovary tissue. Novel peptides appear to perform key roles in N. vitripennis venom function as only four of the highest 15 expressed venom encoding genes are bioinformatically annotationed. The high throughput sequencing data also provided evidence for the existence of an additional 471 novel genes in the Nasonia genome that are expressed in the venom gland and ovary. Finally, metagenomic analysis of venom gland transcripts identified viral transcripts that may play an important part in the N. vitripennis venom function. Conclusions The expression level information provided here for the 79 venom encoding genes provides an unbiased dataset that can be used by the N. vitripennis community to identify high value candidates for further functional characterisation. These candidates represent bioactive peptides that have value in drug development pipelines.

Dataset Information

Functional and evolutionary insights from the genomes of three parasitoid Nasonia species.

Publications

Functional and evolutionary insights from the genomes of three parasitoid Nasonia species.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets