Reconstitution of DNA segregation driven by assembly of a prokaryotic actin homolog.
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ABSTRACT:
SUBMITTER: Garner EC
PROVIDER: S-EPMC2851738 | biostudies-literature | 2007 Mar
REPOSITORIES: biostudies-literature
Publications
Reconstitution of DNA segregation driven by assembly of a prokaryotic actin homolog.
Garner Ethan C EC Campbell Christopher S CS Weibel Douglas B DB Mullins R Dyche RD
Science (New York, N.Y.) 20070301 5816
Multiple unrelated polymer systems have evolved to partition DNA molecules between daughter cells at division. To better understand polymer-driven DNA segregation, we reconstituted the three-component segregation system of the R1 plasmid from purified components. We found that the ParR/parC complex can construct a simple bipolar spindle by binding the ends of ParM filaments, inhibiting dynamic instability, and acting as a ratchet permitting incorporation of new monomers and riding on the elongat ...[more]
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2015-05-01 | GSE45906 | GEO