Ontology highlight
ABSTRACT:
SUBMITTER: Sakurai K
PROVIDER: S-EPMC2853137 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Sakurai Kenji K Shimoji Miho M Tahimic Candice G T CG Aiba Kazuhiro K Kawase Eihachiro E Hasegawa Kouichi K Amagai Yuji Y Suemori Hirofumi H Nakatsuji Norio N
Nucleic acids research 20100113 7
Random integration is one of the more straightforward methods to introduce a transgene into human embryonic stem (ES) cells. However, random integration may result in transgene silencing and altered cell phenotype due to insertional mutagenesis in undefined gene regions. Moreover, reliability of data may be compromised by differences in transgene integration sites when comparing multiple transgenic cell lines. To address these issues, we developed a genetic manipulation strategy based on homolog ...[more]