Identification of novel miRNAs and miRNA dependent developmental shifts of gene expression in Arabidopsis thaliana.

ABSTRACT: microRNAs (miRNAs) are small, endogenous RNAs of 20 approximately 25 nucleotides, processed from stem-loop regions of longer RNA precursors. Plant miRNAs act as negative regulators of target mRNAs predominately by slicing target transcripts, and a number of miRNAs play important roles in development. We analyzed a number of published datasets from Arabidopsis thaliana to characterize novel miRNAs, novel miRNA targets, and miRNA-regulated developmental changes in gene expression. These data include microarray profiling data and small RNA (sRNA) deep sequencing data derived from miRNA biogenesis/transport mutants, microarray profiling data of mRNAs in a developmental series, and computational predictions of conserved genomic stem-loop structures. Our conservative analyses identified five nov

SUBMITTER: Zhan S 

PROVIDER: S-EPMC2854152 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature